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Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with <t>Cy3</t> anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.
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Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with <t>Cy3</t> anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.
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Jackson Immuno af488 donkey anti mouse
Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with <t>Cy3</t> anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.
Af488 Donkey Anti Mouse, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with Cy3 anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Low Prevalence and Inconsistency of LRP4-IgG Detection in Suspected Myasthenia Gravis

doi: 10.1212/NXI.0000000000200554

Figure Lengend Snippet: Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with Cy3 anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.

Article Snippet: For its detection, Cy3 AffiniPure Donkey Anti-Mouse IgG (H + L) (Jackson Immuno Research #715-165-151) was used as the secondary antibody (1:1000).

Techniques: Staining, Expressing, Transfection, Fluorescence, Cell Based Assay